![AffiAB® Anti-HLA Class I Purified [W6/32]](/web/image/product.product/79275/image_1024/%5BAFG-EXB-0305%5D%20AffiAB%C2%AE%20Anti-HLA%20Class%20I%20Purified%20%5BW6-32%5D?unique=1da554d)
Overview
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Specificity:
The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
Antigen
HLA Class I
Clone
W6/32
Species Reactivity
Cow, Non-human primates, Human, Cat
Negative Species
Rabbit
Isotype
Mouse IgG2a
Immunogen
Membrane of human tonsil cells
Application
FC (QC tested), IP, WB, IHC (F), ICC, ELISA, MC (CyTOF)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide