![AffiAB® Anti-HLA-E Purified [MEM-E/06]](/web/image/product.product/79252/image_1024/%5BAFG-EXB-0282%5D%20AffiAB%C2%AE%20Anti-HLA-E%20Purified%20%5BMEM-E-06%5D?unique=1da554d)
Overview
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells) .
Specificity:
The antibody MEM-E/06 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7) . However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Antigen
HLA-E
Clone
MEM-E/06
Species Reactivity
Non-human primates, Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Application
FC (QC tested), IP, IHC (P)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide