![AffiAB® Anti-Hu CD45RB Purified [MEM-143]](/web/image/product.product/79247/image_1024/%5BAFG-EXB-0277%5D%20AffiAB%C2%AE%20Anti-Hu%20CD45RB%20Purified%20%5BMEM-143%5D?unique=d31c23b)
Overview
CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.
Specificity:
The antibody MEM-143 reacts with an extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform) . CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55) . The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.
Antigen
CD45RB
Clone
MEM-143
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Human peripheral blood lymphocytes.
Application
FC (QC tested), IHC (P)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide