![AffiAB® Anti-HLA Class I Purified [MEM-123]](/web/image/product.product/79224/image_1024/%5BAFG-EXB-0254%5D%20AffiAB%C2%AE%20Anti-HLA%20Class%20I%20Purified%20%5BMEM-123%5D?unique=1da554d)
Overview
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Specificity:
The antibody MEM-123 reacts with an extracellular epitope of all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.
Antigen
HLA Class I
Clone
MEM-123
Species Reactivity
Cow, Human, Non-human primates
Negative Species
N/A
Isotype
Mouse IgG3
Immunogen
COS-7 African green monkey kidney cells
Application
FC (QC tested), IP, ELISA
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide