![AffiAB® Anti-HLA-E Purified [MEM-E/02]](/web/image/product.product/79222/image_1024/%5BAFG-EXB-0252%5D%20AffiAB%C2%AE%20Anti-HLA-E%20Purified%20%5BMEM-E-02%5D?unique=1da554d)
Overview
HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells) .
Specificity:
The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.
Antigen
HLA-E
Clone
MEM-E/02
Species Reactivity
Human, Non-human primates
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Recombinant human HLA-E denaturated heavy chain.
Application
WB, IHC (P)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide