![AffiAB® Anti-p21Waf1 Purified [WA-1]](/web/image/product.product/79201/image_1024/%5BAFG-EXB-0231%5D%20AffiAB%C2%AE%20Anti-p21Waf1%20Purified%20%5BWA-1%5D?unique=1da554d)
Overview
The tumour suppressor p21Waf1 (wild-type p53-activated fragment 1; also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.
Specificity:
The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1; intracellular antigen), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.
Antigen
p21Waf1
Clone
WA-1
Species Reactivity
Human, Non-human primates
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Human p21 protein
Application
IHC (P), ICC, ELISA
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide