![AffiAB® Anti-GAPDHS Purified [Hs-8]](/web/image/product.product/79176/image_1024/%5BAFG-EXB-0206%5D%20AffiAB%C2%AE%20Anti-GAPDHS%20Purified%20%5BHs-8%5D?unique=1da554d)
Overview
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1, 3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Specificity:
The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
Antigen
GAPDHS
Clone
Hs-8
Species Reactivity
Pig, Human
Negative Species
N/A
Isotype
Mouse IgM
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Application
WB, ICC, FC (QC tested)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Tris buffered saline (TBS) , pH 8.0, 15 mM sodium azide