![AffiAB® Anti-Can Intra-Acrosomal Protein Purified [Ds-2]](/web/image/product.product/79161/image_1024/%5BAFG-EXB-0191%5D%20AffiAB%C2%AE%20Anti-Can%20Intra-Acrosomal%20Protein%20Purified%20%5BDs-2%5D?unique=1da554d)
Overview
One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Specificity:
The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
Antigen
Intra-Acrosomal Protein
Clone
Ds-2
Species Reactivity
Dog
Negative Species
N/A
Isotype
Mouse IgG
Immunogen
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Application
ICC, WB
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide