Overview
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa) . Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Specificity:
The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa) . Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.
Antigen
Ig Kappa Light Chain
Clone
MEM-09
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Crude thymus membrane fraction.
Application
FC (QC tested), IP, WB, IHC (P), ICC, ELISA
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide