![AffiAB® Anti-HLA Class I Purified [MEM-147]](/web/image/product.product/79133/image_1024/%5BAFG-EXB-0163%5D%20AffiAB%C2%AE%20Anti-HLA%20Class%20I%20Purified%20%5BMEM-147%5D?unique=1da554d)
Overview
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Specificity:
The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting) . MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types.
The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Antigen
HLA Class I
Clone
MEM-147
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
PHA-activated peripheral blood lymphocytes
Application
FC (QC tested), IP, WB
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide