![AffiAB® Anti-CDK1 Purified [POH-1]](/web/image/product.product/79019/image_1024/%5BAFG-EXB-0049%5D%20AffiAB%C2%AE%20Anti-CDK1%20Purified%20%5BPOH-1%5D?unique=1da554d)
Overview
Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
Specificity:
The antibody POH-1 specifically detects Cdk1 (p34Cdc2; an intracellular antigen) . Staining of Cdk1 reflects the proliferating potential of respective tissue.
Antigen
CDK1
Clone
POH-1
Species Reactivity
Cow, Non-human primates, Human
Negative Species
Mouse, Drosophila, Rat, Xenopus
Isotype
Mouse IgG2a
Immunogen
Bacterially expressed full-length human Cdk1 protein
Application
IP, WB, IHC (P), ICC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide