![AffiAB® Anti-Ms MHC Class II Purified Azide Free [M5/114]](/web/image/product.product/78997/image_1024/%5BAFG-EXB-0025%5D%20AffiAB%C2%AE%20Anti-Ms%20MHC%20Class%20II%20Purified%20Azide%20Free%20%5BM5-114%5D?unique=1da554d)
Overview
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Specificity:
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Antigen
MHC Class II
Clone
M5/114
Species Reactivity
Mouse
Negative Species
N/A
Isotype
Rat IgG2b
Immunogen
Activated C57BL/6 mouse spleen cells
Application
FC (QC tested), IP, WB, IHC (P), IHC (F), FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Azide Free
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4