Host
Rabbit
Species Reactivity
Mouse.
Application
WB, IHC, ELISA.
Specificity
This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against
mature 17, 000 MW mouse TNFα and is useful in determining its presence in various assays. The does not
recognize mouse TNFβ (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFα as a 26, 000
protein in immunoblots, particularly in denatured samples. This is also useful for neutralization of mouse
activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended
to incubate the sample with a 1:200 dilution of the for at least 4 hours before being tested. A control of
similarly diluted normal rabbit IgG is recommended..
Shipping Conditions
Ice packs.
Clone
N/A.
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFα
produced in E.coli..
Purity
Purified.
Isotype
IgG.
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid.