Host
Rabbit
Species Reactivity
Human.
Application
WB, IHC, ELISA.
Specificity
This detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (i.e. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The is also useful for neutralization of human IL-6 activity in bioassays.
For neutralization, incubate the sample with a 1:400 dilution of the for at least 4 hours before testing. A
control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in
vitro, this does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a
soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity..
Shipping Conditions
Ice packs.
Clone
N/A.
Immunogen
This was prepared by repeated immunizations with recombinant human IL-6
produced in E.coli..
Purity
Purified.
Isotype
IgG.
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid.