Host
Rabbit
Species Reactivity
Human.
Application
WB, IHC, ELISA, IP, Other.
Specificity
This antiserum has been heated to 56°C for 30 minutes. The antiserum detects
recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-
10 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiple
bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also
useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a
1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit
IgG (heat inactivated) is recommended. The will not neutralize the biological activity of murine IL-10..
Shipping Conditions
Ice packs.
Clone
N/A.
Immunogen
The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-10
produced in E.coli..
Purity
Purified.
Isotype
IgG.
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid.