Host
Rabbit
Species Reactivity
N/A.
Application
WB, IHC, IP.
Specificity
Anti-MBP is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP. .
Shipping Conditions
Ice packs.
Clone
N/A.
Immunogen
MBP epitope tag recombinant protein..
Purity
N/A.
Isotype
IgG.
Storage Conditions
This product is stable for several weeks at 4° C as an undiluted liquid.